First and foremost, check your spelling.  Now, I understand that not everyone is a spelling whiz.  And chances are that if you search this whole help section you'll find a typo somewhere.  But please, please check your spelling.  Run your text through a spell checker or have someone who is a good speller, go through the site and check it.  Most people will excuse one or two errors on a whole site, but repeated errors on a page will turn people off.  Pay special attention to your index or home page as that is probably what people will see first.

Second, have someone who is unfamiliar with your business (and preferably brutally honest) go through your site.  Open the local (on your hard drive, not on a server) copy of your site in your browser and have someone wander through the site.  Go away and let them really look at it, don't hover over their shoulder.  Ask them to take notes on areas where they had questions or weren't sure what to do next.  Ask them to notice if navigation of the site is easy and logical.  Ask them if there are terms on the site that they don't understand.  Let them be honest with you and don't take offense.  Remember, the objective here is to make your site as easy and useful as it can be.  Consider their suggestions and make changes if necessary.  

Third, check all your links.  Make sure everything is complete and works.  Forms and other elements may not work until uploaded.  

Fourth, remember to re-check everything after your site is uploaded.  Sometimes a link may be broken when the upload is performed.  Once uploaded, make sure all forms and elements work.  Just because it worked on your PC doesn't mean that everything is perfect when it's uploaded.  It doesn't hurt to have friends check it on their computer too.  They may spot something you've missed.

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